Separation and purification of Kaxigar Munaka grapevine bleeding sap polysaccharides and analysis of monosaccharide composition

Objective To separate and purify of Kaxigar munaka grapevine bleeding sap polysaccharides and analysis of monosaccharide composition.Methods Prepare crude polysaccharide using alcohol precipitation and by improved Sevage method.Purify the obtained crude polysaccharide by DEAE-52 and Sephadex G-150 column chromatography.Analysis the monosaccharide composition by gas chromatography.Results To get crude polysaccharide by 80% alcohol precipitation and to remove proteins by Sevage method.The crude polysaccharide was separated by DEAE-52,and obtained 8 polysaccharides,respectively GBSK-1,GBSK-2,GBSK-3,GBSK-4,GBSK-5,GBSK-6,GBSK-7and GBSK-8.After being purified,4polysaccharides eluting peaks were unimodal.From the result obtained by GC analysis,monosaccharide composition of GBSK-3 was relatively simple and mainly composed of xylose.The polysaccharides GBSK-2,GBSK-5 had similar monosaccharide composition and they were mainly composed of rhamnose,arabinose,mannose,glucose and galactose.While the polysaccharides GBSK-1was mainly composed of rhamnose,arabinose,xylose,mannose,glucose and galactose.Conclusion Results indicate that this method is suitable for the separation and purification of grapevine bleeding sap polysaccharide.It is suitable to analysis the monosaccharide composition of grapevine bleeding sap polysaccharides by gas chromatography.A scientific basis is provided for the development and utilization of grapevine bleeding sap.