2010•Food ScienceRequires access

Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Diaphragma of Juglans regia L.

Patigul Mahmut

Abstract

The purpose of this work was to isolate and purify polysaccharide from diaphragma of Juglans regia L(PDJL) and analyze its monosaccharide composition.A water-soluble crude polysaccharide was obtained by water extraction,ethanol precipitation and freeze-drying.The crude polysaccharide was purified to obtain a fraction of PDJL-A11 on DEAE-cellulose52 column and SephadexG-100 column.The purity of PDJL-A11 was analyzed by UV spectrophotometer.The monosaccharide composition and molecular ratio of PDJL-A11 were analyzed by gas chromatography.The monosaccharide composition of PDJLA11 was composed of arabinose,fructose,mannose,rhamnose and glucose with a molar ratio of 2.11:6.52:8.81:12.59:15.58.

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Key concepts: Monosaccharide, Polysaccharide, Chemistry, Rhamnose, Ethanol precipitation, Chromatography, Arabinose, Fructose