Effect of Danggui Buxue Decoction on Nuclear Factor Kappa B p65 mRNA Expression in RAW264.7 Cells Stimulated by Oxidized Low-density Lipoprotein

Objective To investigate the influence of serum containing Danggui Buxue Decoction(DBD) on transcription factor of nuclear factor kappa B(NF-κB) activity stimulated by oxidized low-density lipoprotein(Ox-LDL) in RAW264.7 cells,and to explore the cell signal transduction mechanism of DBD in counteracting atherosclerosis(AS).Methods Eight new Zealand rabbits were randomized into 2 groups:blank serum group was given gastric gavage of normal saline,and drug-containing serum group was given DBD(8.4 g·kg-1 ·d-1).After treatment,the rabbits were executed and the serum was separated.RAW264.7 cells were incubated in the culture flask for 24 hours,and then were divided into blank control group,Ox-LDL group,inhibitor group(parthenolide at the concentration of 10 μmol/L),and drug-containing serum group(including 10% volume fraction of serum containing DBD).Except the blank control group,the other groups were stimulated by 100 μg/mL Ox-LDL for 4 hours,and then RAW264.7 cells were collected.NF-κBp65 mRNA expression in RAW264.7 cells was detected with real-time fluorescent quantitative polymerase chain reaction(PCR).Results Ox-LDL group had higher expression of NF-κBp65 mRNA than the blank control group(P 0.05),and the expression of NF-κBp65 mRNA was reduced in drug-containing serum group as compared with that in Ox-LDL group(P ﹤ 0.05).Conclusion Serum containing DBD can down-regulate NF-κBp65 mRNA expression in RAW264.7 cells,and inhibition and blockage of NF-κB p65 mRNA expression may be one of the anti-AS mechanisms of DBD.