The influence of nuclear factor-kappaB on the expression of LOX-1 mRNA and protein in monocytes

Objective To investigate the influence of nuclear factor-kappaB(NF-κB) on the expression of LOX-1 in monocytes in patients with coronary heart disease(CHD).Methods Circulating monocytes of patients with CHD were separated, identified,collected,divided into 3 groups,control group were incubated in RPMI-1640 culture fluid,ox-LDL group were incubated in RPMI-1640 with ox-LDL(40μg/ml)and PDTC group were pretreaf with PDTC(10~(-5)mol/L).Then these cells were collected to isolate the total RNA by the acid guanidium-thiocyanate-phenol-chloroform method and examined the expression of LOX-1 mRNA by RT-PCR.The supernatant liquid of cultured cells was also collected and used to determine the levels of LOX-1 protein by ELISA.Results Compared with control group,the monocyte expression of LOX-1 mRNA increased significantly in ox-LDL group(0.304±0.047 vs 0.813±0.131,P0.05),sLOX-1 protein(ng/ml) in supernatant liquid increased significantly(7.277±1.979 vs 16.517±2.064,P0.05),the LOX-1 mRNA and sLOX-1 protein increased in PDTC group too(0.502±0.140,11.997±1.757,P0.05).Compared with those in ox-LDL group,the expression of LOX- 1 mRNA and content of sLOX-1 protein in supernatant decreased significantly in PDTC group(P0.05).Conclusion ox-LDL can induce the expression of LOX-1 in monocytes,NF-κB mediates the increasing expression of LOX-1 which is induced by ox-LDL in the procedure.