2001•Hereditas (Beijing)Requires access
Effect of Antibiotics on Induction of Callus and Callus Growth in Soybean
Wang Ping
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Abstract
The experiment of inhibiting Agrobacterium LBA4404 was undertaken with 5 antibiotics (the Erythronycin Base,Cefazolin Sodium,Cefradine,2 kinds of Cefotaximes).Among them,Cefotaxime showed the best effect.When Cefotaxime is used in transformation,the ideal concentration is 300mg/L in hypocotyl and 500mg/L in cotyledon node.The response of soybean varieties to Kanamycin is similar in induction of callus rate and is different in brown rate of callus.Differences of the response of soybean explants to Kanamycin were found.The young leaves are sensitive to Kanamycin,but hypocotyl is not.The ideal selecting pressure of Kanamycin is 50-100mg/L in young leaf and cotyledon node,and100-200mg/L in hypocotyl when Kanamycin is used as selection marker.