Response of Phenylalanine Ammonia-lyase(PAL)to Different Inducing Conditions in Wild Soybean(Glycine.soja)

Wild Soybean(Glycine soja) with more desired traits is the ancestor of the cultivated soybean(Glycine max),and phenylalanine ammonia-lyase(PAL)is the key enzyme in the synthesis of isoflavones.With wild soybean(longye 01-177)as material,according to the influencing factors of PAL activities,selecting each 48 h for a cycle and sampling for each 3 h,we studied the enzyme activity,protein content and the variation of enzyme activity under the conditions of low temperature(-4℃)(LT),mechanical damage(MD),ultraviolet ray(UV),hormone(BAP)and normal controls conditions.The results indicated that there was the same change trend of the enzyme activity and protein content of wild soybean PAL under different induction conditions and low temperature had the best induced effect.