2011•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Paeoniflorin,Geniposide and Paeonol in Chaishao Pills by HPLC

Zheng Hui

Abstract

Objective:To establish an HPLC method for the simultaneous determination of paeoniflorin,geniposide and paeonol in Chaishao Pills.Method: Paeoniflorin,geniposide and paeonol were separated on Inertsil ODS-SP(4.6 mm×150 mm,5 μm) column and detected at 230 nm.The mobile phase was acetonitrile-water with gradient elution.The flow rate was 1.0 mL·min-1.Result: Paeoniflorin,geniposide and paeonol were linear within the range of 0.494 4-12.36 μg(r=1),0.492 2-12.305 μg(r=0.999 9) and 0.260 2-6.505 μg,(r=0.999 9) respectively.The average recovery was 98.77%(2.1%),101.64%(1.9%) and 100.92%(1.7%).Conclusion: The method is simple,reproducible,and it can be used for the quality control of Chaishao pills.

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Key concepts: Paeoniflorin, Paeonol, Chromatography, Chemistry, High-performance liquid chromatography, Gradient elution, Linear range, Medicine