Study on Effects of Bufei Decoction on MMP-9 and TIMP-1 in Lung Tissue of Pulmonary Fibrosis Rats

Objective:To observe dynamically the effects of polygonum cuspidate on the expression of tumor necrosis factor-α(TNF-α) and platelet-derived growth factor(PDGF) in the bronchoalveolar lavage fluid(BALF) of pulmonary fibrosis rats and the pathological changes of lung tissue.Methods:96 SD male rats were divided into four groups randomly including control group,model group,prednisone group and Bufei Decoction group(n=24).Except the normal control group,other 3 groups were all adopted bleomycin(BLM) to set up rat model with pulmonary fibrosis by intragastric administration every day,and the materials were draw 7,14,28 days after making models.The contents of MMP-9 and TIMP-1 of lung tissue were detected with with immunohistochemical staining method and the pathological changes of lung tissue were observed with optical microscope.Results:Compared with model group,the degree of alveolitis and pulmonary fibrosis in Bufei Decoction and prednisone group was mild compared with model group,the expressions of MMP-9 and TIMP-1 were decreased significantly in Bufei Decoction and prednisone group.Conclusion:Bufei Decoction can decrease the degree of alveolitis and pulmonary fibrosis decreased significantly in the dexamethasone group and Bufei Decoction could decrease the expressions of MMP-9 and TIMP-1 in lung tissue and reestablish the balance between MMP-9 and TIMP-1,then improve the abnormal metabolism of extracellular matrix,so that it could inhibit the formation of pulmonary fibrosis.