Effects of dexamethasone on expression of glucocorticoid receptor in human monocyte cell line THP-1

Objective To investigate the effects of dexamethasone on the expression of glucocorticoid receptor (GR α, GR β) in human monocyte cell line THP-1.Methods Cultured THP-1 cells were stimulated by dexamethasone with different concentrations and for different durations. The GR α and GR β protein expressions were examined by Western blotting. Results The expressions of GR α and GR β were observed in stimulated and unstimulated THP-1. The quantity of GR β expression was reduced by dexamethasone treatment in a time-dependent manner. The quantity of GR α expression was increased by dexamethasone treatment in time- and dose-dependent manner. Conclusion Dexamethas one stimulation time-dependent reduce GR α expression in THP-1 cell. Dexamethasone stimulation time- dependent and dose-dependent increase GR β expression in THP-I. The expressions of GR a and GR β were regulated by glucocorticoid.