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Effect of the exogenous glucocorticoid on GR expression in fetal rat lung

Li Song

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Abstract

Objective To investigate the effect of glucocorticoid receptor(GR)in late gestation fetal lung around the time of the onset of augmented surfactant production. Methods GR mRNA was measured by Northern blots and hybridizations.GR binding activity was measured by Wrangs method. Results Maternal administration of a high dose dexamethatone increased fetal rat lung GR binding activity,without alteration in GR mRNA levels.Also incubation of fibroblasts (FIB)with 10 -7 mol/L cortisol increased GR immunoreactive protein and binding activity without affecting GR mRNA.Identical treatment, epithelial cells(EPI)were followed by decrease of GR protein without changes of GR mRNA. Conclusion The regulation of GR by glucocorticoid in fetal rat lung occurs at a posttranscriptional level,and increasing circulation glucocorticoid concentration during late gestation may be important to the maturation of fetal lung.

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Objective To investigate the effect of glucocorticoid receptor(GR)in late gestation fetal lung around the time of the onset of augmented surfactant production. Methods GR mRNA was measured by Northern blots and hybridizations.GR binding activity was measured by Wrangs method. Results Maternal administration of a high dose dexamethatone increased fetal rat lung GR binding activity,without alteration in GR mRNA levels.Also incubation of fibroblasts (FIB)with 10 -7 mol/L cortisol increased GR immunoreactive protein and binding activity without affecting GR mRNA.Identical treatment, epithelial cells(EPI)were followed by decrease of GR protein without changes of GR mRNA. Conclusion The regulation of GR by glucocorticoid in fetal rat lung occurs at a posttranscriptional level,and increasing circulation glucocorticoid concentration during late gestation may be important to the maturation of fetal lung.

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Available abstract

Objective To investigate the effect of glucocorticoid receptor(GR)in late gestation fetal lung around the time of the onset of augmented surfactant production. Methods GR mRNA was measured by Northern blots and hybridizations.GR binding activity was measured by Wrangs method. Results Maternal administration of a high dose dexamethatone increased fetal rat lung GR binding activity,without alteration in GR mRNA levels.Also incubation of fibroblasts (FIB)with 10 -7 mol/L cortisol increased GR immunoreactive protein and binding activity without affecting GR mRNA.Identical treatment, epithelial cells(EPI)were followed by decrease of GR protein without changes of GR mRNA. Conclusion The regulation of GR by glucocorticoid in fetal rat lung occurs at a posttranscriptional level,and increasing circulation glucocorticoid concentration during late gestation may be important to the maturation of fetal lung.

Key concepts: Glucocorticoid receptor, Glucocorticoid, Fetus, Internal medicine, Endocrinology, Messenger RNA, Gestation, Lung

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