Effects of Xuebijing injection on ET-1,IL-6 and TNF-α in septic rats

OBJECTIVE To investigate the effects of traditional Chinese medicine Xuebijing injection on ET-1,IL-6 and TNF-α expressions in septic rats.METHODS Sepsis was established in rats by cecum ligation and puncture(CLP).One hundred male Wistar rats were randomly divided into normal control group(group A,N=10),sham operation(group B,N=10),CLP model group(group C,N=40),Xuebijing treated group(group D,N=40).Rats in group C and D were given normal saline or Xuebijing injection with a dose of 2mL·kg-1 intravenously after CLP models were established respectively.Then 10 rats were sacrificed at 3,12,24,and 48 hours after CLP.All rats were measured the serum levels of ET-1,IL-6,and TNF-α through abdominal aortic blood at 3,12,24,48h.RESULTS The rats with sepsis demonstrated an increase in ET1,IL-6,and TNF-α activity of serum.In contrast,the rats after Xuebijing injection treatment exhibited a significant lowering in ET-1,IL-6 and TNF-α activity(P0.05).CONCLUSION Xuebijing injection can effectively reduce ET-1,IL-6 and TNF-α in septic rats,and effectively prevent the further development of sepsis.