DADAG inhibits proliferation and induces apoptosis of L1210 cells in leukaemia

Objective To observe the apoptosis of leukemia L1210 cells in mouse induced by diacetyldianhydrogalactitol(DADAG).Methods The inhibition of L1210 cells proliferation was observed by MTT assay;The apoptosis in L1210 cells was identified by electron microscopy and flow cytometer.Results DADAG inhibited the proliferation of L1210 cells in a concentration-dependent manner.After effecting of DADAG 50.0 mg·L~(-1) on L1210 cells for 24 h,it was observed that the target cells began to shrink and the chromatin began to gather aside through electron microscopy.Apoptotic peaks were detected in the cycle analysis by flow cytometry when DADAG was used for 24 h at the concentration of 12.0,17.2,24.5,35.0 and 50.0 mg·L~(-1).Conclusion DADAG inhibited the proliferation and induced apoptosis of L1210 cells in leukaemia.