The effects of hyperglycemia on the expression of transforming growth factorβ1 and connective tissue growth factor in the liver of rats model induced with carbon tetrachloride

Objective To observe the effects of hyperglycemia on the expression of TGF-β1,CTGF,and to explore hyperglycemia and correlation between hepatic fibrosis. Methods 40 SD rats were randomly divided into four groups according to body weight,n = 10,the control group( C group),hyperglycemia group( H group),liver fibrosis group( M),hyperglycemia + liver fibrosis group( H + M group). The liver fibrosis was made with carbon tetrachloride liver fibrosis model,with the emergence of streptozotocin-induced hyperglycemia. All the animals were killed after eight weeks. The TGF-β1 and CTGF levels were detected by ELISA. The TGF-β1 and CTGF mRNA expression were detected by real-time quantitative RT-PCR. Results TGF-β1,CTGF levels and mRNA expression in group C and H group did not change significantly( P 0.05). Serum levels of these parameters of M and H + M group were significantly higher than that in group C( P 0.05). While the serum these indicators of H + M group increased more significantly( P 0. 05). TGF-β1 and CTGF has a significant positive correlation( r =0. 812,P 0. 05). Conclusion High blood sugar can increase serum liver fibrosis TGF-β1 and CTGF expression and content. The TGF-β1and CTGF content and degree of liver fibrosis has a positive correlation between the evaluation of liver fibrosis. These indexes can be used as an important indicator.