2001•Chinese Journal of Spectroscopy LaboratoryRequires access

Kinetic-Spectrophotometric Determination of Ascorbic Acid in Pharmaceutical Products with Malachite Green-I-Chloramine T

Jianlin Li

Abstract

A new kinetic spectrophotometric method for the determination of ascorbic acid was developed.It is based on the inhibition of the iodine catalysed discoloring reaction of malachite green oxidized by chloramine T with ascorbic acid in hydrochloric acid medium.The linear range of determination is 0—10μg/mL,and the detection limit is 1.2×10 -7 g/mL.The method was used for the determination of ascorbic acid in pharmaceutical products with satisfactory results.The mechanism of the reaction was also discussed.

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Key concepts: Malachite green, Ascorbic acid, Chemistry, Chloramine-T, Detection limit, Hydrochloric acid, Nuclear chemistry, Iodine