EFFECTS OF LIPOPOLYSACCHARIDE ON THE MATURATION OF NOMRAL HUMAN DENDRITIC CELL

Objective: To determine whether bacterial Lipopolysaccharide(LPS) have effects on the phenotype and function of normal human dendritic cell (DC). Methods: Normal human DC were isolated from peripheral blood monounclear cells (PBMC) cultured in granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL) 4 . DC maturation was induced by LPS as experimental groups while the control groups underwent without LPS. DC phenotype was characterized by flow cytometric analysis and the immune function was detected through the stimulatory capacity of allogeneic lymphocytes on the 4th, 8th day of the culture. Result: Normal DC expressed CD1a , CD83 and costimulating molecules CD80 highly but CD14 lowly on cell surface by the stimulating of LPS(P0.05). The quantity of DC and the ability of stimulating allogeneic T lymphocytes were also increased compared with the control groups. Conclusion: Normal human DC cultured in GM-CSF and IL-4 adding with LPS could increase the immune function and maturation of normal human DC. [