Establishment of drug-resistant ovarian cancer cell strain to cisplatin and expression of resistance related genes.

Objective To establish DDP-resistant ovarian cancer cell strain,and investgate expression difference of several drug resistance related genes. Methods Cell line resistant to cisplatin was established from ovarian cancer cell line of SKOV-3 and their biological features were determined.The expressions of several genes associated with drug resistance and P-glycoprotein were measured by RT-PCR and immunohistochemistry. Results The resistance index(RI) of SKOV-3/DDP cell to DDP,fluorouracil,doxorubicin was 13.94,10.83 and 4.52;G0/G1 of SKOV-3/DDP cell line was less than that of SKOV-3 cell line(P0.01),while S,G2/M of SKOV-3/DDP cell line were more than that of SKOV-3 cell line(P0.01).P-glycoprotein positive ratio of SKOV-3/DDP cell line was 65%±5%,while that of SKOV-3 cell line was 6%±2%(P0.001).MDR1、Survivin was positive in SKOV-3/DDP cell line and was negative in SKOV-3/DDP cell line,TopoⅡβ was negative in SKOV-3/DDP cell line and was positive in SKOV-3/DDP cell line. Conclusion Induction of cisplatin resistance in ovarian cancer may lead cross resistance of other drugs which are in different mechanism working on cancer cells,multiple drug-resistance involve several related genes,suggesting more than one pathway leading to resistance transformation.