2013•Xiandai shengwu yixue jinzhanRequires access
Effect of miR-17-92 on Multidrug Resistance of Acute Lymphocytic Leukemia Cell Line
Fengqin Shi, Yamei Xu, Rao Enyu, Yayue Zhang, Wei Ma, Zhao Yong, Xinyi Chen
Open publisher page 0 citations
Abstract
Objective: To investigate the possible role of miR-17-92 in the development of multidrug resistance(MDR)in Acute Lymphocytic Leukemia cell line L1210/DDP.Methods: L1210 cell line resistant to DDP(L1210/DDP) was established.Real-time PCR analysis was used to detect the expression of miR-17-92 between multidrug-resistant acute lymphocytic leukemia cell line L1210/DDP and its parental L1210 cell line.L1210/DDP cells were transfected with the inhibition of miR-17-92(miR-17-92 sponge) or negative control(sponge vector) by Lipofectamine 2000 to down-regulate the expression level of miR-17-92 in L1210/DDP cells.MTS was used to analyze drug sensitivity to DDP and ADR after transfection.Results: miR-17-92 has high expression in multidrug-resistant acute lymphocytic leukemia cell line L1210/DDP.In vitro drug sensitivity assay showed that the down-regulation of miR-17-92 sensitized L1210/DDP cells to anticancer drugs of DDP and ADR.Conclusion: miR-17-92 has high expression in L1210/DDP cell line.Inhibition of miR-17-92 can sensitize L1210/DDP cell to DDP and ADR,reverse drug resistance in part.