The study on the neuroprotective effects of celecoxib against degeneration of dopaminergic neurons

Objective To observe the neuroprotective effects of celecoxib against degeneration of dopaminergic neurons.Methods The primary mesencephalic neural cell deriving from SD newborn rat were cultured.Lipopolysaccharide (LPS) were used to establish inflammator reaction model of PD in vitro.Cultured cells were divided into control group,LPS and CB groups.The concentration of tumor necrosis factor-α(TNF-α) in the culture medium were determined by using ELISA method,and tyrosine hydroxylase immunohistochmisry were performed after cutured for 12 hours and 24 hours. Results The concentration of TNF-α in control group was remarkably lower than in experiment groups at the same time point. ( P 0.01).The concentration of TNF-α in LPS group was highest, respectively:in CB group. The amount of TH-positive cells in control group were more than in experimental groups( P 0.01). The amount of TH-positive cells in LPS group were lowerest,respectively:in CB group.Conclusion Celecoxib have neuroprotective effect through lessening the degeneration of dopaminergic neurons caused by LPS in vitro.