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2011•Zhongguo shouyi xuebaoRequires access

Cloning and sequences analysis of complement C3d cDNA of pig,mouse and duck

Niu Zhong-xiang

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Abstract

The C3d gene from pig,mouse and duck were amplified from total RNA by RT-PCR using primes the designed according to C3d gene sequences of pig,mouse and duck.The cDNA fragments were directly inserted into p MD 18-T plasmid.The recombinant C3d-pMD 18-T was identified by restriction endonucleases digestion and sequencing.Bioinformatics analyses showed that the C3d of mouse had a sequence similarity of 83 %,82 %and 81 % at the amino acid level with pig,mouse and duck C3d respectively,and the C3d of mouse shared 65.8% similarity with duck.This study has a great affect for C3d molecular adjuvant DNA vaccines.

About this research paper

What this paper is about

The C3d gene from pig,mouse and duck were amplified from total RNA by RT-PCR using primes the designed according to C3d gene sequences of pig,mouse and duck.The cDNA fragments were directly inserted into p MD 18-T plasmid.The recombinant C3d-pMD 18-T was identified by restriction endonucleases digestion and sequencing.Bioinformatics analyses showed that the C3d of mouse had a sequence similarity of 83 %,82 %and 81 % at the amino acid level with pig,mouse and duck C3d respectively,and the C3d of mouse shared 65.8% similarity with duck.This study has a great affect for C3d molecular adjuvant DNA vaccines.

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Available abstract

The C3d gene from pig,mouse and duck were amplified from total RNA by RT-PCR using primes the designed according to C3d gene sequences of pig,mouse and duck.The cDNA fragments were directly inserted into p MD 18-T plasmid.The recombinant C3d-pMD 18-T was identified by restriction endonucleases digestion and sequencing.Bioinformatics analyses showed that the C3d of mouse had a sequence similarity of 83 %,82 %and 81 % at the amino acid level with pig,mouse and duck C3d respectively,and the C3d of mouse shared 65.8% similarity with duck.This study has a great affect for C3d molecular adjuvant DNA vaccines.

Key concepts: Biology, Complementary DNA, Molecular biology, Recombinant DNA, Molecular cloning, Restriction enzyme, Cloning (programming), Gene

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