2003•The Chinese Journal of Modern Applied PharmacyRequires access

Influeuce of extraction process on the content of Chlorogenic Acid in Flos Lonnicerae

Bai Hai

Abstract

OBJECTIVE To optimize the extraction process of Chlorogenic acid from Flos Lonicerae. METHOD The optimum extraction was investigated by the orthogonal design and Chlorogenic acid determined by HPLC, the concentration of ethanol, volume of solvent, extraction hour and extraction times were the four factors in the experiment, and three levels of which were chosen to be inspected to optimize the process.RESULTS The concentration of ethanol and extraction times have significance effect on the content of Chlorogenic acid.CONCLUSION The best extraction process is extracting twice with decuple EtOH(70%) and refluxing at 85℃ for 1h each time.

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Key concepts: Chlorogenic acid, Flos, Extraction (chemistry), Chromatography, Chemistry, Solvent, Ethanol, Process (computing)