Molecular Analysis of Plasmid pR_(ST98) from Multidrug Resistant Salmonella typhi by Restriction Endonuclease Diagestion

Objective To Characterize plasmid (pR ST98 ) encoding both resistant to antimicrobial agents and mediating virulence to its host bacteria in S.typhi by restriction endonuclease digestion.Methods Plasmid pR ST98 was isolated and purified by using QIAGEN kit. Nine restriction endonuc leases (BamHI, BglⅡ,EcoRI,EcoRⅤ,HindⅢ,PstⅠ,SacⅠ,SacⅡand SphⅠ) were selected to make the enzyme profile by digestion with pR ST98 DNA.Results The results showed that when using the kit we could get enough and purified plasmid DNA,which was very important in getting an identical restriction pattern. Endonucleases BglⅡ, EcoRⅤ, PstⅠand SacⅡ were especially suitable for mole cular analysis of pR ST98 and were very useful tools for further research.Conclusion The restriction endonuclease pattern of pR ST98 is of much help in character molecular markings, epidemiological surverillence and the location of the functional genes of the plasmid.