Electronic cloning and characterization of sugarcane ScASADH gene using bioinformatics tools

The full-length cDNA sequence of one sugarcane aspartate-semialdehyde dehydrogenase gene(ScASADH gene)was abtained by in silico cloning using EF576477 sequence from Oryza sativa.as the probe sequence. Some characters of the ScASADH gene encoding amino acid, including the composition of amino acid sequence, physical and chemical properties, subcellular localization, transmembrane domain, hydrophobicity/hydrophilicity, secondary and tertiary structure of protein plus functional domains, were analyzed by bioinformatics tools. The results showed that the full-length ScASADH gene from sugarcane was 1 711 bp, including one 1 128bp open reading frame(ORF)which encodes a polypeptide of 375 amino acids. The encoded protein of ScASADH gene, with the presence of signal peptide and several conserved domain sequences, was soluble and located in nucleus, and the corresponding secondary structure of this protein was mainly composed of random coil. The function of the ScASADH protein was mainly involved with translation. Electronic expression analysis revealed that the ScASADH gene was constitutively expressed in the sugarcane root tip, seedling, inflorescence, leaf and stem, and the expression of this gene in the stem of sugarcane was much higher than that in all the other four types of sugarcane tissues.the expression of this gene was regulated under the stresses of Gluconacetobacter and Colletotrichum falcatum.