Electronic cloning and characterization of sugarcane ScCyt C gene using bioinformatics tools

The full-length cDNA sequence of one sugarcane cytochrome C gene( ScCyt C gene) was obtained by in silico cloning using CF576943. 1 sequence from Saccharum sinense as the probe sequence. Some characters of the ScCyt C gene encoding amino acid,including the composition of amino acid sequence,physical and chemical properties,subcellular localization,transmembrane domain,hydrophobicity / hydrophilicity,secondary and tertiary structure of protein plus functional domains,were analyzed by bioinformatics tools. The results showed that the full-length ScCyt C gene from sugarcane is 1073 bp which encodes a polypeptide of 112 amino acids. The encoded protein of ScCyt C gene,with a conserved domain sequences,is soluble and located in cytoplasm or chloroplast,and the corresponding secondary structure of this protein is mainly composed of random coil. The function of the ScCyt C protein is mainly involved with translation. It is highly conservative in different plants. Electronic expression analysis revealed that the ScCyt C gene is constitutively expressed in all the types of sugarcane tissues,and the expression of this gene in the stem and root of sugarcane is much higher than that in all the other types of sugarcane tissues. In addition,the expression of this gene is regulated under the stress of low temperature. The results obtained in this study will provide reference for molecular cloning,structural analysis and function identification of ScCyt C gene in sugarcane.