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Determination of jatrorrhizine,palmatine and berberine in Cortex phellodendron by HPLC

Ding Qing, De-ran Xu

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Abstract

To separate and determine jatrorrhizine,palmatine and berberine in Cortex phellodendri:HPLC method was developed.ODS column was used,and the mobile phase consisted of 5%(w/v)SDS-Britton-Robinson(pH 2.5)-acetonitrile-triethylamine(0.5∶56∶34∶0.5).The detecting wavelength was 345 nm.Jatrorrhizine,palmatine and berberine can be separated completely.The method can be used for the research of Cortex phellodendri and Chinese traditional medicine containing berberine.

About this research paper

What this paper is about

To separate and determine jatrorrhizine,palmatine and berberine in Cortex phellodendri:HPLC method was developed.ODS column was used,and the mobile phase consisted of 5%(w/v)SDS-Britton-Robinson(pH 2.5)-acetonitrile-triethylamine(0.5∶56∶34∶0.5).The detecting wavelength was 345 nm.Jatrorrhizine,palmatine and berberine can be separated completely.The method can be used for the research of Cortex phellodendri and Chinese traditional medicine containing berberine.

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Available abstract

To separate and determine jatrorrhizine,palmatine and berberine in Cortex phellodendri:HPLC method was developed.ODS column was used,and the mobile phase consisted of 5%(w/v)SDS-Britton-Robinson(pH 2.5)-acetonitrile-triethylamine(0.5∶56∶34∶0.5).The detecting wavelength was 345 nm.Jatrorrhizine,palmatine and berberine can be separated completely.The method can be used for the research of Cortex phellodendri and Chinese traditional medicine containing berberine.

Key concepts: Jatrorrhizine, Palmatine, Berberine, Chromatography, Chemistry, High-performance liquid chromatography, Biochemistry

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