Protective effect of ischemic preconditioning on liver ischemia/reperfusion injury in rats with cirrhosis

Objective To investigate the protective effect of ischemic preconditioning(IP) on liver ischemia/reperfusion(I/R)injury in rats with cirrhosis.Methods Thirty-two SD rats with liver cirrhosis induced by carbon tetrachloride were randomly divided into IP group and Non-IP(NIP) group,according to whether an IP was performed before liver I/R injury or not.The function of liver, superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malonyldialdehyde(MDA),adenine nucleotide(ATP,ADP,AMP),energy charge(EC),nitric oxide(NO)in liver tissues,the changes in liver histology,and one-week survival rate after the test in both groups were compared.Results Compared with NIP group,the serum AST,ALT ,and LDH and the MDA contents significantly decreased(P0.05,P0.001,P0.001P0.05,respectively);the liver contents of SOD(P0.05),GSH-PX(P0.01),ATP(P0.01) significantly increased in IP group.Light and electron microscope showed that in IP group the degree of liver injury was much less than that in NIP group.The postoperative 1 week survival rate in IP group also improved,but there was no statistical difference between IP group and NIP group(P=0.069).Conclusions IP exerts a protective effect on I/R injury of cirrhotic liver in rats.