Isolation,identification and degrading characteristics of a high effective oil-degrading bacterial strain

Taking the bean oil as only the carbon source,oil-degrading strain was isolated from the sewage water of the restaurant through enrichment,domestication culture,elected plate separation and fermentation.It was identified by morphological,physiological and biochemical characteristics,and 16SrDNA sequence homology analysis.Effects of culture time,temperature and different oil on degrading rate were studied.Lipase and biosurfactant activities were detected.The results indicated that 6 strains of oil-degrading bacteria were isolated,and strain DX2-6 showed the highest degrading rate.The strain was identified as Staphylococcus saprophyticus which showed great environmental adaptation with degrading rate above 66.2% at 10g/L bean oil after a 48h cultivation at 20～40 ℃.The maximum degrading rate achieved 84.7% at 30℃.The strain showed high capability of degrading different edible oils and had better lipase and biosurfactant activities.It is suggested that DX2-6 is a high effective oil-degrading strain and has a good application prospect for treatment of oil wastewater.