Ditermination of diltiazem hydrochloride for injection by internal Standard

[Objective] To establish an HPLC method of determination of diltiazem hydrochloride.[Methods] Chromatographic conditions included Diamonsil ODS C18 column was used with 50℃ column temperature and a mobile phase of α-caphor-sulfonic acid-sodium acetate buffer-solution-acetonitrile-methanol(25∶40∶35)at a flow rate of 1.2 mL/min and 240 nm detection,and phenyl benzoate was used as internal standard.[Results] The linear of diltiazem hydrochloride was 0.1007～0.5035 mg/mL,the recovery was 97.85%,RSD=0.799%(n=9).[Conclusions] This methods is simple,rapid,accurate and reliable.