Study on HPLC Fingerprint Chromatogram of Selaginella tamariscina and Selaginella pulvinate and Amentoflavone Determination

Objective To establish a method to analyze HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate and to determine the content of amentoflavone.Methods HPLC was applied for establishment of fingerprints of Selaginell atamariscina and Selaginella pulvinate which from different origin to analyze their fingerprint characteristic and content of amentoflavone.Results The different characteristic seven peaks in the chromatogram consisted of the common pattern of Herba Selaginella tamariscina and Selaginlla pulvinate.Two important mutual fingerprint peaks were found in both of them,one of which is amentoflavone.The content of amentoflavone in Herba Selaginella tamariscina and Selaginella pulvinate was 1.77～3.82% and 0.45～0.85% separately.Conclusion The HPLC fingerprint chromatogram of Selaginella tamariscina and Selaginella pulvinate was similar,but their were very different in the content of amentoflavone.