Chemical Study on the Water Soluble Polysaccharide from Spores of Ganoderma lucidum

The polysaccharide isolated from the spores of Ganoderma lucidum was purified and characterized.The crude polysaccharide was obtained from the hot-water extract of the spores of Ganoderma lucidum and the polysaccharide SGL-Ⅱ-2 was isolated by Sepharose CL-6B column chromatography.The HPLC analysis indicated that the SGL-Ⅱ-2 was a homogeneous polysaccharide.Its molecular weight was estimated to be 5.37×10 4.By means of partial hydrolysis with acid,periodate oxidation,Smith degradation,methylation,IR,GC,GC-MS and 13 C NMR,the structure of SGL-Ⅱ-2 was identified.It is a lowly branched molecule with basic structure composed of (1→3)-linked-glucose and (1→6)-linked-glucose residues in the main chain.Some of (1→6)-linked-glucose residues are substituted at 3-O or 4-O.The side chain is composed of (1→4)-linked-galactose.The nonreduced end is glucose.