The function of HR-HPV and LCT test and histology biopsy with colposcopy of cervical in diagnosis of cervical lesion

Objective To evaluate the function and application of high risk human papillomavirus (HR-HPV) test, liquidbased cytology test (LCT) and histological biopsy with colposcopy. Methods 601 patients with abnormal LCT results in the outpatient department of gynecology from January 2009 to October 2011 were reexamined using HR-HPV test. The results of HR-HPV and LCT tests were compared with histological biopsy under colposcope. Results 67.2% (404/601) of the patients were found to be HR-HPV positive. The infection rate of patients with ASC-US, LSIL, ASC-H, HSIL and CA was 45.5%, 66.2%, 74.4%, 89.1% and 100.0%, respectively, and the difference was significant (P0.05). The sensitivity of diagnosing CIN and cervical lesions in the combined LCT and HR-HPV test was 86.1%. The result was higher than the LCT test (77.1%) and HR-HPV test (72.0%) (P0.05). The false negative rate (13.9%) was lower than the LCT and HR-HPV test (P0.05). The compliance rate was 50.8% (102/201) between LSIL with LCT and CIN with histology biopsy. The compliance rate was 83.3% (333/400) between≥LSIL with LCT and ≥CIN with histology biopsy. 88.0% (24/25) in cancer among 601 patients with abnormal LCT. Among the 201 cases of ASC, 102 cases (50.7%) had normal or cervical inflammation, 22 cases (11.0%) had CINⅠ, 40 cases (19.9%) had CINⅡ , 34 cases (16.9%) had CINⅢ , and 2 cases (1.0%) had cervical cancer. In the HR-HPV test, the detection rate was 59.2% (119/201); with a sensitivity of 71.7% (71/99), specficity of 53.0% (54/102) and an accuracy of 64.7%. Conclusion The pathogenic development of cervical lesion is correlated closely with HR-HPV infection. LCT and HR-HPV tests, together with histological examination can be used to improve the diagnosis cervical lesion.