Expression of the matrix metalloproteinases 7 and 9 in the prostate cancer

Objective To investigate the expression of the matrix metalloproteinases(MMP)-7 and-9 in the pro-state cancer,and the relationship of between the MMP and vascular endothelial growth factor(VEGF).Methods The expression of the MMP 7 and 9 were detected by immunohistochemistry assay and morphology quantitative analysis technique.At the same time,the PC3 M cells were cultured,the expression of MMP-9 and the relationship between MMP-9 and VEGF were detected with Boyden chamber and zymography assay.Results The expression of the MMP-7 and MMP-9 were different in each graduation of prostate cancer(P0.01).The result of the Boyden chamber showed that the number of the penetration Matrigel decreased gradually in different group [PC3MPC3M+MMP-9Ab(1∶100)PC3M+ MMP-9Ab(1∶50)].The result of zymography assay showed that the expression of MMP-9 and MMP-2 were stable in the PC3M cells cultured in the culture dishes,but in the VEGF co-cultured culture dishes,the content of the MMP-9 was elevated dose-dependently.Conclusion MMP7 and MMP-9 can be used as the indicators of prostate cancer invasion and metastasis,and the VEDF was related with the secretion of the MMPs.