Gene Expression Profile Study on the Cerebral Gene in Model Mouse with Kidney-yang Deficiency Treated by Kidney Tonifying Drugs

Objective:To inquire into the cerebral gene change of the model mouse with kidney-yang deficiency caused by excessive physical and sexual activities and its improved syndrome change due to using drugs of efficacy such as Jinkuishenqiwan and Youguiwan which reinforcing the kidney-yang according to traditional Chinese medicine,the mechanism of the medicine. Methods:There were four groups randomly:the control group which has 10 male mice,the model group which has 15 male mice,the Jinkuishenqiwan treatment group which has 10 male mice and the Youguiwan group which has 10 male mice. All of them were fed normally,and perfused with 0. 5mL of distill water for each mouse in the control group and model group,and with 0. 5mL sus-pension of the drugs for each in the treatment groups every day. The experimental mice in the model group and the treatment groups were kept with six female mice each in the same cage,and all of the male mice swam until they submerged and scooped up from water everyday,which lasted four weeks. The mice might induce the kidney-yang deficiency because of excessive physical and sexual activities. The brain gene of the mice between control group,model group and treatment groups was detected with a mouse brain gene chip of 36K Mouse genome array,which screened the differentially expressed genes according to the ratio equal to or above 2 and equal to or below 0. 5 with the related fluorescent intensity comparing with the two groups',part of which could be further verified by means of qRT-PCR. Results:The animal model of kidney-yang deficiency in the model group was induced by way of excessive physical and sexual activities successfully,there were twenty three genes among the up-regulated genes in the model group versus control group but down-regulated in the treatment group versus model group,mainly involving the related genes of inflammation/immunization and neurotransmissions/signal transduction. There were six genes among the down-regulated genes in the model group versus control group but up-regulated in the treatment group versus model group,mainly including the related genes of cellular cycle and structure,neurotransmissions/signal transduction and transcription. Six differentially expressed genes,such as GH,Scgb3a1 and so on were verified with qRT-PCR in the groups. Conclusion:Jikuishenqiwan and Youguiwan may make it markedly up -regulated to notably down-regulated genes of hormone and melanin-concentrating hormone( MCH) for model of mice of kidney-yang deficiency,and promote cellular proliferation,which can inquire into effect mechanism of the two drugs and their differences in genetic level at the same time.