Effects of Sucrose and PEG-4000 on Pollen Germnation and Pollen Tube Growth of Pyrus pyrifolia cv. Whangkeumbae in Vitro

The effects of different concentrations of sucrose and PEG-4000 on pollen germination and pollen tube growth of Pyrus pyrifolia(Burm.F.)Nakai cv.Whangkeumbae were studied.The results showed that appropriate amount of sucrose and PEG-4000 was conductive to the pollen germination and the pollen tube growth.But when the concentration was too high,it inhibited pollen germination and pollen tube growth.When the sucrose concentration was 100 g/L,the pollen germination rate and pollen tube length reached 41.3%and 693μm respectively.When the PEG-4000 concentration was 15 g/L,the pollen germination rate and pollen tube length reached 39.9%and 675μm respectively.The concentration of 50 mg/L H3BO4,10 mg/L MgSO4,100 g/L sucrose and 15 g/L PEG-4000 was suitable to pollen germination and pollen tube growth.