The effect of sites and dosage in olfactory ensheathing cells (OECs) transplantation on curing dated spinal cord injury

Objective: To explore the effect of sites and dosage in olfactory ensheathing cells(OECs) transplantation on curing dated spinal cord injury.Methods:OECs were purified from human olfactory bulb and cultured for 1 or 2 weeks in the laboratory of our hospital.The spinal dural mater was conventionally cut through the dorsal approach of vertebrae.50ul OECs suspension were transplanted under microscope to one sites in the border of the superior part of the injured spinal cord and the normal spinal cord(30 cases in Group A).To transplant 50ul×2 OECs(at most 50ul×4) to several sites in the either side of the injured spinal cord(the border of injured spinal cord and normal spinal cord that approach to the normal spinal cord) with the same method(30 cases in Group B).Results: To evaluate the 106 cases of injured spinal cord who accepted the OECs transplantation in our hospital in 2~4 weeks after the operation with ASIA.The cases have different improvements in sense,movement,defecating,urinating and function of vegetative nerve.The improvements of the cases in Group B are more obvious than Group A.Conclusions: Transplanting 50ul×2 OECs(at most 50ul×4) to several sites in the either side of the injured spinal cord is superior to the therapy that transplanting 50 ul OECs to one sites in the superior part of the injured spinal cord.