Multidrug resistance glioma cell line C6/adr and the reversal of MDR

Objective To investigate the mechanism of multidrug resistance (MDR) in human glioma cell and the way to overcome MDR. Methods The MDR cell line C6/adr was established.The expression of mdr 1 gene and its P glyco protein(P gp) in the C6/adr cell lines were observed by RT PCR and immunohistochemical staining. And the reversal of MDR by verapamil, erythromycin, dihydropyridine, P gp monclonal antibody, and dansen were studied with MTT assay or HPLCA.Results Mdr 1 gene of C6/adr cell line was positive, which overexpressed P gp. The drug resistance of C6/adr cell line coule be partly reversed by 2～6μg/ml of verapamil or 50～100μg/ml of erythromycin, 5μg/ml of dihydropyridine. As concentration in crease, they had better results. Among these drugs, 100μg/ml of erythromycin. Among these drugs, 100μg/ml of erythromycin had the best results.1μg/ml of dihydrpyridine, P gp monoclone antibody, dansen had no effect.Conclusions Mdr l gene and its D gp expression might be in association with the MDR of glioma cells.Verapamil, erythromycin and dihydropyridine could reverse MDR of glioma cells.