Studies on the factors affecting for establishing diabetic model in mice by intraperitoneal injection of Alloxan

Objective: To investigate the factors affecting for establishing diabetic model in mice by intraperitoneal injection of Alloxan.Methods: The effects of the gender,body weight of mice,the dose,times of administration and different solvents were observed in order to evaluate the model successful rate,mortality and fasting blood glucose by intraperitoneal injection of Alloxan in mice.Results: The experiment results showed that the male was more suitable for being induced diabetic model than the female.The dose was the main factor.The model successful rate and the mortality were increasing with the increasing of dose.The solution of Alloxan in buffer was more available than that in physiological saline to induce diabetic mice.Conclusion: A better diabetic mice modle can be gained by intraperitoneal injection of Alloxan with 185 mg/kg once after being fasted twelve hours.The diabetic model in mice are more stabile.