The Separation and Purification of Total Flavonoids from Prunella Vulgaris L. by Macroporous Resin

The separating and purifying purpose of the flavonoids from Prunella vulgaris L.was studied and compared with four kinds of macroporous resins including D101,AB-8,NKA-Ⅱand NKA-9.The adsorption rate,desorption rate and adsorption kinetics of each resin were studied,and some technical conditions were sptimized via static and dynamic experiments.The results showed that the weak polar AB-8 macroporous resin was good sorbent of the flavonoids,its adsorption rate and desorption rate were 90.29% and 81.29%,respectively.The optimum conditions for separating and purifying the flavonoids by AB-8 resin were:injecting concentration 3.732 mg·mL-1,pH=5,injecting velocity 2.0 mL·min-1,40%(v/v) alcohol as desorption solvent,desorption velocity of flow 1.5 mL·min-1 and elution volume 5 BV.The content of flavonoids in the purified extracts reached 81.58% from about 46.25%% in the crude extracts after AB-8 resin in the optimized conditions.