Detection and clinical significance of S-100B protein and neuron-specific enolase in serum of newborns with Hypoxic-ischemic encephalopathy

Objective: To study the changes o f serum concentrations of S-100B protein and Neuron-specific enolase(NSE) in newborns with hypoxic-ischemic encephalopathy(HIE),the correlation between S-100B protein and NSE,and between S-100B protein and NSE and neonatal behavioral neurological assessment(NBNA),and clinical significance.Methods: Thirty-five term newborns with HIE were divided into three groups,mild HIE group(n = 10),moderate HIE group(n = 15) and severe HIE group(n = 10).Twenty-five term normal newborns were served as control group.Serum concentrations of S-100B protein and NSE were measured by enzyme-linked immunosorbent assay(ELISA).Results: One day after birth,the level of S-100B protein and NSE in HIE neonate were much higher than normal neonate.The more serious condition,the higher was for S-100B protein and NSE(F = 149.4、214.8,P = 0.00).The control group for blood serum S-100B protein and NSE level had no obvious change(t = 0.956,0.858,P 0.05) for first 3 days after born,d1 after born was slightly higher for mild HIE group,and drop dramatically after 3 days,the difference had great significance(t = 6.78,4.586,P ﹤ 0.001);d1 after born was slightly higher for moderate HIE group,and drop after 3 days,the difference had statistics significance(t = 2.255,2.515,P ﹤ 0.05);the blood serum S-100B protein and NSE level maintained in high level for severe HIE group within 3 days after born,the difference had no statistics significance(t = 1.015,0.789,P 0.05),and there was a significant positive correlation between S-100B and NSE(r = 0.923,P = 0.000),there was a significant negative correlation between S-100B and NBNA(r =-0.656,P = 0.000),and there was a negative correlation between NSE and NBNA(r =-0.479,P = 0.001).Conclusion: The levels of S-100B protein and NSE in moderate and severe HIE infants were approximately higher than those in control group,and they were identical with the 520— severity of HIE,so they may be useful markers for the diagnosis of HIE.The measurement of S-100B protein and NSE will be very important to the early diagnosis and judgment of therapeutic efficacy.