Study on Differentiation of Rat Adipose Tissue-derived Mesenchymal Stem Cells Into Hepatic Lineage in Vitro

Objective:To observe whether SD rat adipose tissue-derived mesenchymal stem cells(ADMSCs)can differentiate into hepatic lineage in vitro.Methods:Adipose-derived stem cells were isolated,subcultivated,and evaluated for cell surface marker expres- sion using immunofluorescence.Hepatocyte growth factor(HGF)and fibroblast growth factor-4(FGF-4)were used to induce the ADM- SCs into hepatic lineage.The morphology change was observed by inverted microscope.14 and 21 days after induction,albumin as a hepatic special marker was detected by immunofluorescence.And cell culture media at the indicated times after induction were collect- ed,and urea nitrogen was detected by urea nitrogen detection kit.Results:Adipose tissue-derived cells expressed CD29 strongly,but not CD31 and CD45.Since 14 days after induction,Hepatocyte-like morphology was observed.Albumin can be detected by im- munofluorescence.Urea nitrogen detection showed that differentiated ADMSCs had the function of urea production in a time-dependent manner.Conclusions:Mesenchymal stem cells derived from rat adipose tissue can be induced into hepatic lineage which has parts of hepatocytes’features on morphology,phenotype and function.