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2015•Chinese Journal of Clinical Obstetrics and GynecologyRequires access

Expression of TNF-αand MSTN in placenta tissue of preeclampsia patients

Zhang Xiao-l

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Abstract

Objective To detect the relationship of the expression of TNF alpha and MSTN in placenta tissue and preeclampsia.Methods 40 cases of full-term healthy maternal(normal group),40 cases of mild preeclampsia women(experimental group A)and 40 cases of severe preeclampsia women(experimental group B)were recruited in this study.Expression and location of TNF alpha and MSTN in the placenta tissue were determined by immunohistochemical SP method,semi-quantitative PCR(RT-PCR)and protein imprinting methods(Western blot).Results TNF alpha and MSTN expressed in all the three groups and the location in placenta is basically the same.Expression levels of TNF alpha and MSTN mRNA was significantly higher in group A and group B compared to control group(P0.01)and they were significantly higher in Group B than in group A(P0.01).Conclusion The changes of the molecular biology of MSTN and TNF-αmay be one of the mechanism of preeclampsia.

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What this paper is about

Objective To detect the relationship of the expression of TNF alpha and MSTN in placenta tissue and preeclampsia.Methods 40 cases of full-term healthy maternal(normal group),40 cases of mild preeclampsia women(experimental group A)and 40 cases of severe preeclampsia women(experimental group B)were recruited in this study.Expression and location of TNF alpha and MSTN in the placenta tissue were determined by immunohistochemical SP method,semi-quantitative PCR(RT-PCR)and protein imprinting methods(Western blot).Results TNF alpha and MSTN expressed in all the three groups and the location in placenta is basically the same.Expression levels of TNF alpha and MSTN mRNA was significantly higher in group A and group B compared to control group(P0.01)and they were significantly higher in Group B than in group A(P0.01).Conclusion The changes of the molecular biology of MSTN and TNF-αmay be one of the mechanism of preeclampsia.

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Available abstract

Objective To detect the relationship of the expression of TNF alpha and MSTN in placenta tissue and preeclampsia.Methods 40 cases of full-term healthy maternal(normal group),40 cases of mild preeclampsia women(experimental group A)and 40 cases of severe preeclampsia women(experimental group B)were recruited in this study.Expression and location of TNF alpha and MSTN in the placenta tissue were determined by immunohistochemical SP method,semi-quantitative PCR(RT-PCR)and protein imprinting methods(Western blot).Results TNF alpha and MSTN expressed in all the three groups and the location in placenta is basically the same.Expression levels of TNF alpha and MSTN mRNA was significantly higher in group A and group B compared to control group(P0.01)and they were significantly higher in Group B than in group A(P0.01).Conclusion The changes of the molecular biology of MSTN and TNF-αmay be one of the mechanism of preeclampsia.

Key concepts: Preeclampsia, Placenta, Medicine, Immunohistochemistry, Western blot, Tumor necrosis factor alpha, Andrology, Real-time polymerase chain reaction

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