Expression and role of AQP9 in models of cultured steatosis hepatocytes

Objective:To explore the expression and role of AQP9 in the models of cultured steatosis hepatocytes.Methods:Steatosis models of hepatocytes was established by adding oleic acid to the growing L-02 cell strain.The expression of AQP9 mRNA was measured with RT–PCR and the protein expression of AQP9 were measured by Western blotting.Lipid droplets in the hepatocytes were observed by oil red staining and the contents of triglyceride,FFA and glycerol in hepatocytes were measured with analyzed kit.Results:Through oil red staining.a few lipid droplets were observed at 24h and steatosis hepatocyte increased greatly at 72h.Triglyceride,FFA and glycerol contents in hepatocytes of model groups also increased,as compared with the control group(P0.01).In model groups,mRNA expression of AQP9 increased at 24h,and elevated remarkably at 72h as compared with the normal control group(P0.05),and protein expression of AQP9 increased at 24h,and elevated remarkably at 72h compared with the control group(P0.05).Conclusion:The overexpression of AQP9 in model groups could lead to disturbance of lipid metabolism and probably AQP9 participates the process of steatosis of hepatocytes.