Parameter analysis and methodologic evaluation of reticulocyte detected by flow cytometry

Objective To observe the superiority of reticulocyte detection by the flow cytometry (FCM) to tradition method and the connection of its detected information with clinic.Methods Reticulocyte was determined by the flow cytometry with thiazole orange as marker,the percentage of reticulocyte,reticulated platelet and intensity of RNA remaind fluorencence were detected tradition method used microscope.Results The repeatability and accuracy had no significant differences between the methods of flow cytometry and tradition,but in the flow cytometry coefficient of variation was lower than that in the tradition method.In 300 objects of the anaemia group,fluorencence intensity and percentage of reticulocyte had significant difference compared with health group (P0.05).In 100 cases of thrombocytopeina,fluorencence intensity and percentage of reticulated platelet had significant difference compared with the health group (P0.05).Conclusion Compared with the tradition method,the flow cytometry has the advantages of good repeatability and high accuracy,which can simultaneously determine the average fluorencence intensity of reticulocyte and reticulated platelet,the average flnorencence intensity of reticulocyte could reflect their maturity.