Effects of diammonii glycyrrhizinatis on acute lung injury induced by cardiopulmonary bypass

Objective Diammonii glycyrrhizinatis was shown to exert a protective effect on myocardium against ischemia-reperfusion injury. The purpose of this study was to evaluate the effects of diammonii glycyrrhizinatis on acute lung injury induced by cardiopulmonary bypass(CPB), Methods Twenty-four ASA Ⅱ - Ⅲ patients (9 male, 15 female) aged 20-60 yr, weighing 45-75 kg scheduled for elective value replacement were divided into two groups at random: control group (group C, n = 12) and diammonii glycyrrhizinatis group (group G, n = 12) . Patients with infections diseases or immunodiflciency and those who were taking corticosteroid and drugs which may affect immune function were excluded. Anesthesia was induced and maintained with midazolam, fentanyl and vecuronium. In group G diammonii 2.5 mg·kg-1 10% glucose 50 ml was infused during the interval between intubation and skin incision. Blood samples were taken from arterial line at 10 min after induction (T0), 30 min after start of CPB (T1 ), 30 min after aortic unclamping (T2), at the end of surgery (T3), 4 h and 24 h after operation (T5, 6) for determination of plasma TNT-α IL-β, IL-10, MDA levels and Mn-SOD activity. Respiratory index (RI) PA-aDO2/PaO2 ] was calculated at 10 min after induction of anesthesia (T0), 10 min and 30 min after CPB (T1, 2) and at the end of surgery. Results There was no significant difference in age body weight, CPB time and aortic cross clamping time between the two groups. The TNF-α, IL-β, IL-10, MDA levels and Mn-SOD activity were not significanttly different at T0 between the two groups, and increased significanttly after start of CPB in both groups ( P 0.05 or 0.01). But TNF-a level was significantly lower at T, and T2 in group G than in group C (P 0.05). IL-1β level was significantly lower at T1,3 in group G than in group C (P 0.01 or 0.05). IL-10 level was significantly higher at T1,3 and T5 in group G than group C ( P 0.05 or 0.01). The plasma MDA level in group G was significantly lower at T2,4 than that in the control group (P 0.05) . Mn-SOD activity was significantly higher at T1, 2 in group G than that in group C ( P 0.05). RI was significantly lower at T1 and T3 in group G than in group C ( P 0.05) .Conclusion Diammonii glycyrrhizinatis has auti-iuflammatory and oxygen free radical reducing effects and could protect the lungs from acute injury indued by CPB.