Expression analysis of wheat chitinase gene Cht4 in the resistance and susceptible varieties to cereal cyst nematode

Expression analysis about wheat chitinase gene,in the different growing stages and different positions of different resistance cultivars after CCN inoculation,was carried by semi-Q RT-PCR,using wheat Actin gene as a control.The result showed that the quantity of chitinase gene expression obvious increased after 12 h inoculation,and there were no obvious differences between resistant and susceptible cultivars.After 36 h inoculation,the expression quantity in both cultivars increased obviously,and the quantity in resistant cultivars was more than susceptible cultivars,and in leaves was more than in roots.After 60 h inoculation,the gene expression quantity in resistant and susceptible cultivars was keeping a high level,and the former was more than the latter.The expression in susceptible cultivars after 84 h inoculation didn't have obvious difference from the ones without inoculation,but the expression quantity in resistant cultivars was still higher than those without inoculation.