The separation of berberine and palmatine from the cortex ofPhellodendron chinense Schneid using high speedcountercurrent chromatography

To separate alkaloids from the cortex of Phellodendron chinense Schneid. using high speed countercurrent chromatography . High speed countercurrent chromatography using chloroform methanol∶0.2 mol/l dilute hydrochloric acid (2∶1∶1) as solvent system,stationary phase:upper phase,mobile phase:lower phase, flow-rate 2.0 ml/min,revolution speed: 800 r/min. sample size:200 mg. 20.4 mg Palmatine(fraction Ⅰ) and 64.8 mg Berberine(fraction Ⅱ) were separated and determined by HPLC which fraction Ⅰ and Ⅱ were in 99% pure and identified by IR、()~1H-NMR and MS. High speed countercurrent chromatography can separate alkaloids from the cortex of Phellodendron chinense Schneid. quickly.