The Hypolipidemic Effect of Different Component Combination of Catsia tora, Salvia miltiorrhiza,Ilex kudingcha and Gynostemma pentaphyllum

Objective: To study the effects of combination of Catsia tora,Salvia miltiorrhiza and Ilex kudingcha(compositeⅠ) and Combination of C.tora,S.miltiorrhiza and Gynostemma pentaphyllum(compositeⅡ) on the levels of serum lipids and antioxidant activity in hyperlipidemic mice,as well as comparison of the synergistic reaction of two different combinations in hyperlipidemic mice.Method: Fifty male Kunming mice were randomly divided into normal control,high-fat control,positive control group,compositeⅠand compositeⅡ group,each consisting of 10 animals.In addition to normal group which was fed with normal diet,other four groups were fed with high-fat diet for 4 weeks to establish the experimental hyperlipemia model.Then the normal control and high-fat control were given distilled water orally,positive control group was given simvastatin at the dose of 7 mg·kg-1,compositeⅠgroup was given C.tora(1 596 mg·kg-1),S.miltiorrhiza(197 mg·kg-1) and I.kudingcha(230 mg·kg-1) gavage,compositeⅡ group was given C.tora(1 596 mg·kg-1),S.miltiorrhiza(197 mg·kg-1) and G.pentaphyllum(648 mg·kg-1)gavage.Daily intragastrical administrations of traditional Chinese medicine combination for 4 weeks consecutive days were on the experimental groups mice.The effects of two combination groups on body weight,serum levels of total cholesterol(TC),triglycerides(TG),high density lipoprotein cholesterol(HDL-C),malondildehyde(MDA) and the activity of superoxide dismutase(SOD) were measured.The pathological changes of liver,kidney,spleen tissues stained with Hematoxylin-eosin(HE) were observed under light microscope.Result: Compared with the hyperlipidemic model group,both combination groupss could significantly decreased the level of serum TC,TG,MDA,and remarkably increased the concentration of serum HDL-C and the activity of SOD in the hyperlipemia mice(P0.01),and compositeⅠhad a stronger effects on reducing the level of serum TC and MDA than compositeⅡ.Conclusion: Two combination groups have the potent,hypolipidemic effect and liver protective effects,and could improve the antioxidant activity.Furthermore,the effect of compositeⅠis more remarkable.