Construction and Expression of Eukaryotic Expression Vector of Mouse IL-5

[Objective] The study aimed to clone and construct the eukaryotic expression plasmids containing mouse IL-5 cDNA and investigate whether they expressing in 293 cells.[Methods] Total RNA was extracted from mouse spleen to amplify mouse inerleukin-5(mIL-5) cDNA by reverse transcription-polymerase chain reaction(RT-PCR).After sequence analysis,mIL-5 was cloned into expression vector Fc-pRc-CMV.The recombinant plasmids were transfected into 293 cells and the expression of target gene was detected by RT-PCR and ELISA.[Result] The inserted DNA sequence in Fc-pRc-CMV was identical to mIL-5 cDNA,which was verified correctly by sequencing.The corresponding gene expression was detected in recombinant plasmid transfected 293 cells.[Conclusion] The mouse IL-5 cDNA was successfully cloned and its eukaryotic expression plasmid was constructed.