Eukaryotic expression and identification of human Slit2 gene in HEK-293 cell line

Objective:To construct the recombinant eukaryotic expression vector for human Slit2 full-length cDNA and express it in human embryonic kidney cells (HEK-293 cell line). Methods:The full length cDNA of hSlit2 was obtained and cloned into eukaryotic expression vector pCMV-GFP-C to construct the recombinant eukaryotic expression vector pCMV-GFP-C/hSlit2,and the recombinant plasmid was transfected into 293 cells by calcium phosphate coprecipitation. The expression of hSlit2 protein was analyzed by Western blot. Results:The eukaryotic expression recombinant plasmid pCMV-GFP-C / hSlit2 was successfully constructed and confirmed by restriction enzyme digestion,and the expression of hSlit2 cDNA was detected by Western blot. Conclusion:The recombinant eukaryotic expression vector is correctly constructed,and the study offers an experimental base for the study of the fuction of hSlit2 protein and its proteolytic fragments in central nervous system.