Purification Technology of Total Alkaloids from Rhizoma Coptidis

Objective To optimize the best purification process of total alkaloids from Rhizoma Coptidis.Methods The factors that affect the result of purification to total alkaloids were determined.Results The optimum isolation process was as follows,adding 10 time of 0.5% sulphuric acid into Rhizoma Coptidis extract,adjusting to pH 9 by lime cream,standing for 2 hours,filtering,adjusting to pH 1 by hydrochloric acid,adjusting the salinity of solution up to 10% by sodium chloride,standing for 2 hours,washing the precipitation by distilled water to pH 5.Conclusion The optimum purification technology of total alkaloids from Rhizoma Coptidis is simple,stable and feasible.