Construction of human cytomegalovirus pp150 gene prokaryotic expression clones and its mechanism

[Objective] To construct human cytomegalovirus pp150 gene prokaryotic expression clones and produce recombinant antigen for serodiagnosis of HCMV infection.[Methods] One fragment encoding pp150 antigen epitopes(495-691) were amplified by PCR and inserted into prokaryotic expression vector pET-11a,transferred into the host bacteria BL21 and induced to express pp150 protein,then identified by western blot.[Results] Expression vector pp150-pET-11a clones were expressed effectively and its relative molecular weight was 21.5kD analysed by SDS-PAGE,and its product was 16.38% in the total protein,the expressed protein was analysed by Western blot.[Conclusions] The pp150 protein is expressed effectively in the BL21strains and lays foundation for further application.